Journal: Animal Nutrition

Article Title: Protective impacts of berberine in weaned piglets fed without zinc oxide: Insight of jejunal barrier function, water absorption, and gut microbiota and metabolism

doi: 10.1016/j.aninu.2025.05.012

Figure Lengend Snippet: Effects of dietary berberine supplementation on the expression of intestinal inflammatory cytokine, barrier function proteins and aquaporins and ion transporters in weaned piglets. (A) The mRNA relative expression levels of TJP1 , OCLN , CLDN1 , MUC2 , and MUC4 . (B) The mRNA relative expression levels of IL10 , IL1B , IL6 , TNF , IL17A , RELA , and NFKBIA . (C) The mRNA relative expression levels of AQP1 , AQP3 , AQP7 , AQP8 , AQP10 , AQP11 , SLC12A2 , and SLC9A3 . (D) The protein relative expression levels of TJP1, CLDN1, OCLN, RELA, and P-RELA. CON = basal diet; XBJ = basal diet + 50 mg/kg berberine; ZIN = basal diet + 1600 mg/kg zinc oxide (d 0–14) or 110 mg/kg zinc oxide (d 15–28). Asterisks indicate significance levels: ∗ for P < 0.05, ∗∗ for P < 0.01, and ∗∗∗ for P < 0.001, n = 6.

Article Snippet: These suppliers provided the primary antibodies used: zonula occludens-1 (21773e1-AP), heat shock protein family A member 6 (13616-1-AP), heat shock protein family A member 8 (10654-1-AP), and nuclear factor kappa B p65 (66535-1-IG) from Proteintech Group, Inc., Wuhan, Hubei, China; phosphorylated nuclear factor kappa B p65 (bs-5662R), occludin (bs-10011R) and claudin-1 (bs-0790R) from Beijing Biosynthesis Biotechnology Co., Ltd. , Beijing, China.

Techniques: Expressing

Journal: Animal Nutrition

Article Title: Protective impacts of berberine in weaned piglets fed without zinc oxide: Insight of jejunal barrier function, water absorption, and gut microbiota and metabolism

doi: 10.1016/j.aninu.2025.05.012

Figure Lengend Snippet: Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes (DEGs). (A-C) KEGG enrichment analysis was performed on the DEGs identified in comparisons between the XBJ and CON groups, the ZIN and CON groups, and the XBJ and ZIN groups. (D) KEGG enrichment analysis of 96 co-expressed DEGs in XBJ vs. CON and XBJ vs. ZIN groups. IgA = immunoglobulin A; ABC = ATP-binding cassette; PD-L1 = programmed death-ligand 1; PD-1 = programmed cell death protein 1; NF-kappa B = nuclear factor kappa-light-chain-enhancer of activated B cells; Th1 = T-helper 1; Th2 = T-helper 2; DNA = deoxyribonucleic acid; IL17 = interleukin 17; P450 = cytochrome P450; CAMP = cyclic adenosine monophosphate. CON = basal diet; XBJ = basal diet + 50 mg/kg berberine; ZIN = basal diet + 1600 mg/kg zinc oxide (d 0–14) or 110 mg/kg zinc oxide (d 15–28). n = 6.

Article Snippet: These suppliers provided the primary antibodies used: zonula occludens-1 (21773e1-AP), heat shock protein family A member 6 (13616-1-AP), heat shock protein family A member 8 (10654-1-AP), and nuclear factor kappa B p65 (66535-1-IG) from Proteintech Group, Inc., Wuhan, Hubei, China; phosphorylated nuclear factor kappa B p65 (bs-5662R), occludin (bs-10011R) and claudin-1 (bs-0790R) from Beijing Biosynthesis Biotechnology Co., Ltd. , Beijing, China.

Techniques: Binding Assay

Journal: Chinese Medicine

Article Title: Ershen Dan attenuates atherosclerosis by modulating the NOTCH1/NF-κB/NLRP3 signaling pathway to suppress M1 macrophage polarization

doi: 10.1186/s13020-025-01232-9

Figure Lengend Snippet: ESD inhibits the NOTCH1/NF-κB/NLRP3 signaling pathway and delays the progression of atherosclerosis. A Western blotting analysis was used to detect the protein expression of NOTCH1 and HES1. B , C Analysis of NOTCH1 and HES1 protein expression. D Protein expression of NLRP3, NF-κB p65, and p-NF-κB p65. E , F Expression analysis of NLRP3, NF-κB p65, and p-NF-κB p65. G Immunohistochemical detection of CD86 expression in the aorta of mice, scale: 200 μm. H Immunohistochemical statistical analysis (n = 3). Statistical data are expressed as mean ± SD. In comparison with the control group, * P < 0.05, *** P < 0.001; in comparison with the model group, # P < 0.05, ## P < 0.01, ### P < 0.001; in comparison with the RSV group, & P < 0.05

Article Snippet: Neurogenic locus notch homolog protein 1 (NOTCH1; 10062-2-AP; Proteintech, China), hairy and enhancer of split-1 (HES1; A0925; ABclonal, China), NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3; 30109-1-AP; Proteintech, China), nuclear factor (NF)-κB p65 (10745-1-AP; Proteintech, China), phospho-NF-κB p65 (82335-1-RR; Proteintech, China), and NADPH oxidase 2 (NOX2; 19013-AP; Proteintech, China) were used.

Techniques: Western Blot, Expressing, Immunohistochemical staining, Comparison, Control

Journal: Chinese Medicine

Article Title: Ershen Dan attenuates atherosclerosis by modulating the NOTCH1/NF-κB/NLRP3 signaling pathway to suppress M1 macrophage polarization

doi: 10.1186/s13020-025-01232-9

Figure Lengend Snippet: Effects of key components of ESD on M1-type macrophages. A The CCK-8 assay was used to detect the cell survival rate after modeling. B The CCK-8 assay was also used to detect the cell survival rate of the model after drug pre-protection. C Western blotting analysis was used to detect the protein expression levels of COX-2 and iNOS. D , E Protein expression analysis of COX2 and iNOS (n = 3). F COX-2 immunofluorescence staining. Red: COX-2; Blue: DAPI. Scale: 200 μm (n = 3). G iNOS immunofluorescence staining. Green: iNOS; Blue: DAPI. scale: 200 μm (n = 3). H ROS expression in each group (n = 5), scale: 100 μm. I Expression of P65 and p-p65 proteins. J – L Analysis of P65 and p-p65 proteins (n = 3). M Expression of NF-κB mRNA (n = 3). Statistical data are expressed as mean ± SD. In comparison with the control group, * P < 0.05, and in comparison with the model group, # P < 0.05

Article Snippet: Neurogenic locus notch homolog protein 1 (NOTCH1; 10062-2-AP; Proteintech, China), hairy and enhancer of split-1 (HES1; A0925; ABclonal, China), NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3; 30109-1-AP; Proteintech, China), nuclear factor (NF)-κB p65 (10745-1-AP; Proteintech, China), phospho-NF-κB p65 (82335-1-RR; Proteintech, China), and NADPH oxidase 2 (NOX2; 19013-AP; Proteintech, China) were used.

Techniques: CCK-8 Assay, Western Blot, Expressing, Immunofluorescence, Staining, Comparison, Control